Total bacterial and species-specific 16S rDNA micro-array quantification of complex samples
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of Brachyspira species based on biochemical scheme and 16S rDNA partial sequencing
Avian intestinal spirochetosis (AIS) is a disease of birdscharacterized by a marked colonization of the cecum and/or rectumwith anaerobic intestinal bacteria of the genus Brachyspira. Thepurpose of the study was to determine the occurrence of Brachyspiraspp from avian sources in Iran and to characterize selected isolates bybiochemical and molecular methods. Out of 165 fecal samples obtained fro...
متن کاملSequence-based identification of Mycobacterium species using the MicroSeq 500 16S rDNA bacterial identification system.
We evaluated the MicroSeq 500 16S rDNA Bacterial Sequencing Kit (PE Applied Biosystems), a 500-bp sequence-based identification system, for its ability to identify clinical Mycobacterium isolates. The organism identity was determined by comparing the 16S rDNA sequence to the MicroSeq database, which consists primarily of type strain sequences. A total of 113 isolates (18 different species), pre...
متن کاملFigure 3. 5’-16S rDNA and ITS multiple alignments of all observed mycobacterial species sequevars 16S rDNA
C A T G C A A G T C G A A C G G A A A G G C C T C T T C G G A G G T A C T C G A G T G G C G A A C G G G T G A G T A A C M. avium sqv. I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M. avium ssp. avium sqv. II . . . . . . . . . . . . . . . . . . . . . . . C . . . . . . G . . . . . . . . . . . . . . . . . . . . . . . . . ....
متن کاملAssignment of Reference 5’-end 16S rDNA Sequences and Species-Specific Sequence Polymorphisms Improves Species Identification of Nocardia
16S rDNA sequence analysis is the most accurate method for definitive species identification of nocardiae. However, conflicting results can be found due to sequence errors in gene databases. This study tested the feasibility of species identification of Nocardia by partial (5'-end 606-bp) 16S rDNA sequencing, based on sequence comparison with "reference" sequences of well-annotated strains. Thi...
متن کاملHigh-throughput sequencing of 16S rDNA amplicons characterizes bacterial composition in cerebrospinal fluid samples from patients with purulent meningitis
Purulent meningitis (PM) is a severe infectious disease that is associated with high rates of morbidity and mortality. It has been recognized that bacterial infection is a major contributing factor to the pathogenesis of PM. However, there is a lack of information on the bacterial composition in PM, due to the low positive rate of cerebrospinal fluid bacterial culture. Herein, we aimed to discr...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2006
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2006.02805.x